The Establishment of A Temporary Expression System in Chicken Oviduct Epithelium

In the development of techniques by using a bioreactor to produce a special protein, and the establishment of a temporary expression system from primary cell culture of special tissue to analyze the expression regulators and the recombinant gene will greatly expedite the progress of research. This paper is the first report on the establishment of a temporary expression system from the primary culture of chicken oviduct epithelium, which continuously synthesized and secreted ovalbumin(OA) during more than two weeks of incubation. When the secretion function of the cells decreased, it could be restored in most of the cells by adding hormones in the culture medium within one week of incubation. The OA can be quickly and easily measured by using dotimmuofiltration assay in the cell culture medium because it is a kind of secretary protein. In order to examine whether the exogenous genes can be expressed in the primary culture cells, the green fluorescent protein gene(GFPG) was transfected into oviduct cells, and green fluorescence was observed in the cytoplasm of the epithelial cells. These results indicate that the temporary expression system in chicken oviduct epithelium is an effective and convenient system to analyze the expression regulators and the recombinant gene.